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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
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- Product number
- PA5-54482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DNAI1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: APHKQPHKQS ISIGRGTRKR DEDSGTEVGE GTDEWAQSKA TVRPPDQLEL TDAELKEEFT RILTANNPHA PQNIVRYSFK EGTYKPIGFV NQLAVHYTQV Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 80%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNAI1 in control (vector only transfected HEK293T lysate) and dNAI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DNAI1 Polyclonal Antibody (Product # PA5-54482).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DNAI1 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a DNAI1 Polyclonal Antibody (Product # PA5-54482).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human testis tissue shows distinct cytoplasmic positivity in cells of the seminiferus ducts. Samples were probed using a DNAI1 Polyclonal Antibody (Product # PA5-54482).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human fallopian tube and colon tissues using DNAI1 Polyclonal Antibody (Product # PA5-54482). Corresponding DNAI1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human fallopian tube, kidney, lymph node and testis using DNAI1 Polyclonal Antibody (Product # PA5-54482) (A) shows similar protein distribution across tissues to an independent DNAI1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human fallopian tube using DNAI1 Polyclonal Antibody (Product # PA5-54482) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human colon using DNAI1 Polyclonal Antibody (Product # PA5-54482) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human kidney using DNAI1 Polyclonal Antibody (Product # PA5-54482).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human testis using DNAI1 Polyclonal Antibody (Product # PA5-54482).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNAI1 in human lymph node using DNAI1 Polyclonal Antibody (Product # PA5-54482).
