Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-84466 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84466, RRID:AB_11026198
- Product name
- Rabbit Polyclonal DNAI1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human DNAI1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway.
Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.
Gomi K, Arbelaez V, Crystal RG, Walters MS
PloS one 2015;10(2):e0116507
PloS one 2015;10(2):e0116507
Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.
Walters MS, Gomi K, Ashbridge B, Moore MA, Arbelaez V, Heldrich J, Ding BS, Rafii S, Staudt MR, Crystal RG
Respiratory research 2013 Dec 3;14:135
Respiratory research 2013 Dec 3;14:135
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNAI1 Antibody [NBP1-84466] - Analysis in control (vector only transfected HEK293T lysate) and dNAI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human colon shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human fallopian tube shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human prostate shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human fallopian tube, placenta, prostate and testis using Anti-DNAI1 antibody NBP1-84466 (A) shows similar protein distribution across tissues to independent antibody NBP1-84465 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining in human testis and prostate tissues using NBP1-84466 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.