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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA308951 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ROCK1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 0.68mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell extract. B. 30 ug C2Cl2 whole cell extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ROCK1 antibody [N1N2], N-term detects ROCK1 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:500
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: A431 . B: H1299. C: Molt-4 . 5% SDS PAGE. TA308951 diluted at 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using ROCK1(TA308951) antibody at 1:200 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ROCK1 antibody immunoprecipitates ROCK1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of ROCK1 by 3 ug of ROCK1 antibody (TA308951) 5% SDS-PAGE The immunoprecipi
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD1 xenograft , using ROCK1(TA308951) antibody at 1:500 dilution.
- Validation comment
- IHC
