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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00006093-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006093-M01, RRID:AB_547801
- Product name
- Mouse Monoclonal ROCK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ROCK1 - Rho-associated, coiled-coil containing protein kinase 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A novel role of Rho-kinase in the regulation of ligand-induced phosphorylated EGFR endocytosis via the early/late endocytic pathway in human fibrosarcoma cells.
Integration of virtual screening with high-throughput screening for the identification of novel Rho-kinase I inhibitors.
Nishimura Y, Bereczky B, Yoshioka K, Taniguchi S, Itoh K
Journal of molecular histology 2011 Oct;42(5):427-42
Journal of molecular histology 2011 Oct;42(5):427-42
Integration of virtual screening with high-throughput screening for the identification of novel Rho-kinase I inhibitors.
Gong LL, Fang LH, Peng JH, Liu AL, Du GH
Journal of biotechnology 2010 Feb 1;145(3):295-303
Journal of biotechnology 2010 Feb 1;145(3):295-303
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK1 Antibody (2E2) [H00006093-M01] - ROCK1 monoclonal antibody (M01), clone 2E2 Analysis of ROCK1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ROCK1 Antibody (2E2.) [H00006093-M01] - ROCK1 Antibody (2E2) [H00006093-M01] - Detection limit for recombinant GST tagged ROCK1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ROCK1 Antibody (2E2) [H00006093-M01] - Analysis of monoclonal antibody to ROCK1 on HeLa cell. Antibody concentration 10 ug/ml.
