Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M00722 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ROCK1 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for ROCK 1/ROCK1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat ROCK 1/ROCK1 information: Molecular Weight: 158175 MW; Subcellular Localization: Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Cytoplasm, cytoskeleton . Cell membrane . Cell projection, lamellipodium . Cell projection, ruffle . Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes; Tissue Specificity: Detected in blood platelets.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- CDE-18
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway.
RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells.
High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway.
Dietary Calcium Alleviates Fluorine-Induced Liver Injury in Rats by Mitochondrial Apoptosis Pathway.
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction.
Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling.
β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice.
ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat.
Huang X, Lei S, Xiong X, Wang X, Zhao L, Wang N, Wan N, Li B
Chemistry & biodiversity 2024 Feb;21(2):e202301383
Chemistry & biodiversity 2024 Feb;21(2):e202301383
RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells.
Dong Q, Luo Y, Yin Y, Ma Y, Yu Y, Wang L, Yang H, Pan Y, Zhang D
Journal of cellular and molecular medicine 2023 Aug;27(15):2123-2135
Journal of cellular and molecular medicine 2023 Aug;27(15):2123-2135
High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway.
Zhao H, Kong H, Wang W, Chen T, Zhang Y, Zhu J, Feng D, Cui Y
Investigative ophthalmology & visual science 2022 Jul 8;63(8):22
Investigative ophthalmology & visual science 2022 Jul 8;63(8):22
Dietary Calcium Alleviates Fluorine-Induced Liver Injury in Rats by Mitochondrial Apoptosis Pathway.
Li H, Hao Z, Wang L, Yang J, Zhao Y, Cheng X, Yuan H, Wang J
Biological trace element research 2022 Jan;200(1):271-280
Biological trace element research 2022 Jan;200(1):271-280
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction.
Wu D, Chen X, Wang L, Chen F, Cen H, Shi L
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Aug;116:108836
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Aug;116:108836
Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling.
Gong J, Guan L, Tian P, Li C, Zhang Y
Medical science monitor : international medical journal of experimental and clinical research 2018 May 27;24:3514-3523
Medical science monitor : international medical journal of experimental and clinical research 2018 May 27;24:3514-3523
β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice.
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu DH, Deng W, Lai Y, Zhou JH, Zhang SX, Lin WW, Chen DF
Brain research 2014 Mar 13;1552:41-54
Brain research 2014 Mar 13;1552:41-54
ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat.
Ma J, Liang S, Wang Z, Zhang L, Jiang J, Zheng J, Yu L, Zheng X, Wang R, Zhu D
Journal of cellular physiology 2010 Jan;222(1):82-94
Journal of cellular physiology 2010 Jan;222(1):82-94
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney, using ROCK1 Antibody(M00722)ROCK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROCK1 Antibody (M00722)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image