21850-1-AP

antibody from Invitrogen Antibodies

Targeting: ROCK1 p160ROCK

Antibody data

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Validation data

Product number: 21850-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: ROCK1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: SNRRYLSSA NPNDNRTSSN ADKSLQESLQ KTIYKLEEQL HNEMQLKDEM EQKCRTSNIK LDKIMKELDE EGNQRRNLES TVSQIEKEKM LLQHRINEYQ RKAEQENEKR RNVENEVSTL KNQLEDLKKV SQNSQLANEK LSQLQKQLEE ANDLLRTESD TAVRLRKSHT EMSKSISQLE SLNRELQERN RILENSKSQT DKDYYQLQAI LEAERRDRGH DSEMIGDLQA RITSLQEEVK HLKHNLEKVE GERKEAQDML NHSEKEKNNL EIDLNYKLKS LQQRLEQEVN EHKVTKARLT DKHQSIEEAK S (401-710 aa encoded by BC113114)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.35 mg/mL
Storage: -20°C

ROCK1 protein structure - 21850-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.