Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- Ab125629 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant ROCK1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human ROCK1 Antibody, Recombinant, could be used for WB and so on.ApplicationWB: 1/500-1/1000Protein FunctionProtein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1344-1354
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
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- Aladdin Scientific (provider)
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- Experimental details
- Recombinant ROCK1 Antibody (Ab125629) - Western Blot All lanes: Recombinant ROCK1 Antibody (Ab125629) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 158 kDa Observed band size: 173 kDa Exposure time: 10.0 s