RQ7415

antibody from NSJ Bioreagents

Targeting: ROCK1 p160ROCK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7415

Provider

NSJ Bioreagents

Product name

Rho-associated protein kinase 1 Antibody / ROCK1

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Rho-associated protein kinase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) rat liver, 4) rat C6, 5) mouse liver and 6) mouse NIH 3T3 cell lysate with Rho-associated protein kinase 1 antibody. Predicted molecular weight ~158 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-2 OS cells with Rho-associated protein kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rho-associated protein kinase 1 antibody.