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LearnPA1-26351
antibody from Invitrogen Antibodies
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA1-26351 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aurora A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-26351 detects Aurora A from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation.
Guarino Almeida E, Renaudin X, Venkitaraman AR
Nucleic acids research 2020 Aug 20;48(14):7844-7855
Nucleic acids research 2020 Aug 20;48(14):7844-7855
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS- 30 µg lysates) of HCT 116 (Lane 1), HCT 116 treated with Paclitaxe l(100nM for 18 hrs) (Lane 2), HT-29 (Lane 3), HT-29 treated with Paclitaxel (100nM, 18hrs) (Lane 4), SK-BR-3 (Lane 5) and SK-BR-3 treated with Nocodazole (100ng/mL, 16 hrs) (Lane 6). The blot was probed with Anti-Aurora A Polyclonal Antibody (Product # PA1-26351, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 48 kDa band corresponding to AuroraA were observed across the cell lines tested, with an increase in expression upon treatment.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Aurora A was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Aurora A Polyclonal Antibody (Product # PA1-26351) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Mitotic chromosomes (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitotic cells with AuroraA expression concentrated at the spindle poles. Panel e represents the resting cells showing cytoplasmic expression of AuroraA . Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of S35 labeled recombinant Aurora A (5.0 mg) using Aurora A polyclonal antibody (Product # PA1-26351) followed by gel electrophoresis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP analysis of 35S-Met-labeled whole cell lysate transfected with a human Aurora A expression construct using (Product # PA1-26351) Aurora A Polyclonal Antibody.IP reaction : 2-10 µg/mg lysate.
