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LearnEB05035
antibody from Everest Biotech
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- EB05035 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB05035, RRID:AB_2290113
- Product name
- Goat anti-Aurora kinase A / AURKA Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-Aurora kinase A / AURKA Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-QNKESASKQS- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB05035 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB05035 in the right panel (vector-only transfection in first and fourth lanes).
