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antibody from R&D Systems
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF3295 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Aurora A Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Aurora A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Aurora B and rhAurora C is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
O14965- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Aurora A in HeLa Human Cell Line. Aurora A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Aurora A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3295) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to mitotic spindles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
