Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF503 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL16 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CXCL16 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL16, rhFractalkine, recombinant mouse Fractalkine, and recombinant rat Fractalkine is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BSU2
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-33 modulates inflammatory brain injury but exacerbates systemic immunosuppression following ischemic stroke.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
Zhang SR, Piepke M, Chu HX, Broughton BR, Shim R, Wong CH, Lee S, Evans MA, Vinh A, Sakkal S, Arumugam TV, Magnus T, Huber S, Gelderblom M, Drummond GR, Sobey CG, Kim HA
JCI insight 2018 Sep 20;3(18)
JCI insight 2018 Sep 20;3(18)
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7166-75
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7166-75
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7166-75
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7166-75
Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
Arthritis and rheumatism 2007 Mar;56(3):949-59
Arthritis and rheumatism 2007 Mar;56(3):949-59
Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
Journal of leukocyte biology 2007 Jan;81(1):176-85
Journal of leukocyte biology 2007 Jan;81(1):176-85
Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK 3rd, Woods JM, Koch AE
Arthritis and rheumatism 2006 Aug;54(8):2402-14
Arthritis and rheumatism 2006 Aug;54(8):2402-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF503). The ND50 is typically 0.1-0.4 µg/mL.