MA5-27845

antibody from Invitrogen Antibodies

Targeting: CXCL16 CXCLG16, SR-PSOX, SRPSOX

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27845

Provider

Invitrogen Antibodies

Product name

CXCL16 Monoclonal Antibody (GT516)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: HeLa, HeLa membrane extract

Antibody clone number

GT516

Concentration

1 mg/mL

References

Submitted references

Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells.

Vella JL, Molodtsov A, Angeles CV, Branchini BR, Turk MJ, Huang YH
Life science alliance 2021 Oct;4(10)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CXCL16 in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with CXCL16 monoclonal antibody (Product # MA5-27845) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CXCL16 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) at a dilution of 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845). HeLa whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCL16 Monoclonal Antibody (GT516) detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. CD11c + cells express CXCL16 in melanoma-associated vitiligo (MAV)-affected skin. (A) Heat map showing gene expression of chemokines and receptors in CD11c + CD11b neg , CD11c + CD11b + , and CD8 + T cells sorted from skin of MAV-affected mice. Data presented as log2-normalized expression. (B) Surface expression of CXCL16 on CD11c + cells isolated from skin of naive (n = 3) or MAV-affected (n = 5) mice, skin-draining LNs, or spleen 7, 14, and 35 d after surgery. Representative of three independent experiments. (C) Surface expression of CXCL16 on CD45 + CD11c + , CD45 + CD11c neg , and CD45 neg cells isolated from skin of MAV-affected mice, 35 d after surgery. Representative of two independent experiments with skin collected between 35- and 60-d post-surgery. Dotted horizontal line indicates background CXCL16 expression in unstained cell sample. (D) Expression of CXCL16 and CD11c in unaffected and MAV-affected mice; CD11c (green), CXCL16 (red), and nuclei (blue). Scale bar, 50 mum. (E) CXCL16 expression in IF images from skin of unaffected and MAV-affected mice; n = 5-8 mice. (F) CXCL16 expression in hair follicles with or without CD11c + cell clusters in MAV-affected skin; n = 8 mice. (G) CD11c + and CXCL16 + cells in skin from MAV-affected patients. Stains identify CXCL16 (green) and CD11c (red); dark red identifies colocalization of CD11c and CXCL16. Scale bar, 25 mum. (H) Percent of CD11c + cells expressing CXCL16 in MAV-affected patient skin. (B, C, H) Symbols r