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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-7961 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7961, RRID:AB_627242
- Product name
- Anti-CDK6
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen.
Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2.
Modulation of microRNA processing by p53
p27Kip1 inhibits cyclin D-cyclin-dependent kinase 4 by two independent modes.
Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.
Cimica V, Smith ME, Zhang Z, Mathur D, Mani S, Kalpana GV
BMC cancer 2010 Nov 19;10:634
BMC cancer 2010 Nov 19;10:634
Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2.
Yang G, Chang B, Yang F, Guo X, Cai KQ, Xiao XS, Wang H, Sen S, Hung MC, Mills GB, Chang S, Multani AS, Mercado-Uribe I, Liu J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jun 15;16(12):3171-81
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jun 15;16(12):3171-81
Modulation of microRNA processing by p53
Hiroshi I. Suzuki, Kaoru Yamagata, Koichi Sugimoto, Takashi Iwamoto, Shigeaki Kato, Kohei Miyazono
Nature 2009 Jul;460(7254):529-533
Nature 2009 Jul;460(7254):529-533
p27Kip1 inhibits cyclin D-cyclin-dependent kinase 4 by two independent modes.
Ray A, James MK, Larochelle S, Fisher RP, Blain SW
Molecular and cellular biology 2009 Feb;29(4):986-99
Molecular and cellular biology 2009 Feb;29(4):986-99
Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.
James MK, Ray A, Leznova D, Blain SW
Molecular and cellular biology 2008 Jan;28(1):498-510
Molecular and cellular biology 2008 Jan;28(1):498-510
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 37
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human bone marrow shows strong cytoplasmic and nuclear positivity in bone marrow poietic cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
