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Learn14052-1-AP
antibody from Invitrogen Antibodies
Targeting: CDK6
PLSTIRE
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14052-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEKDGLCRA DQQYECVAEI GEGAYGKVFK ARDLKNGGRF VALKRVRVQT GEEGMPLSTI REVAVLRHLE TFEHPNVVRL FDVCTVSRTD RETKLTLVFE HVDQDLTTYL DKVPEPGVPT ETIKDMMFQL LRGLDFLHSH RVVHRDLKPQ NILVTSSGQI KLADFGLARI YSFQMALTSV VVTLWYRAPE VLLQSSYATP VDLWSVGCIF AEMFRRKPLF RGSSDVDQLG KILDVIGLPG EEDWPRDVAL PRQAFHSKSA QPIEKFVTDI DELGKDLLLK CLTFNPAKRI SAYSALSHPY FQDLERCKEN LDSHLPPSQN TSELNTA (1-326 aa encoded by BC027989)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of CDK6 antibody (14052-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 14052-1-AP ( CDK6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C2C12 cells (shRNA samples) were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14052-1-AP (CDK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human CDK6 (Product # 14052-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CDK6 (IP:14052-1-AP, 4ug; Detection:14052-1-AP 1:1000) with Jurkat cells lysate 2880 ug.
