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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX111588 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111588, RRID:AB_1951612
- Product name
- Raf1 antibody [N1C1-2]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references IL-1β-Induced Mesenchymal Stem Cell Migration Involves MLCK Activation via PKC Signaling.
Lin CY, Zu CH, Yang CC, Tsai PJ, Shyu JF, Chen CP, Weng ZC, Chen TH, Wang HS
Cell transplantation 2015;24(10):2011-28
Cell transplantation 2015;24(10):2011-28
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX111588 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [N1C1-2] (GTX111588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Raf1(GTX111588) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Raf1(GTX111588) antibody at 1:500 dilution.
