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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS060 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-Raf-1 (S301) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat, and Xenopus ~73 kDa Raf-1 in Western blots.
- Reactivity
- Bovine, Canine, Chicken/Avian, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detects Phospho-Raf-1 (S301) by Western Blot. Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the approximately 73 kDa Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l PPase). The blot is identical to the control except that it was incubated in l PPase (1200 units for 30 minutes) before being exposed to the anti-phospho-Raf-1 (S301). The immunolabeling of Raf-1 is completely eliminated by treatment with l PPase.
