F54689

antibody from NSJ Bioreagents

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54689

Provider

NSJ Bioreagents

Product name

Raf1 Antibody / c-Raf

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the c-Raf antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse Neuro-2a cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Jurkat cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse skeletal muscle tissue with c-Raf antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse Neuro-2a cells with c-Raf antibody; Left=isotype control, Right= c-Raf antibody.