F54952

antibody from NSJ Bioreagents

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54952

Provider

NSJ Bioreagents

Product name

Raf1 Antibody / c-Raf

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Rabbit

Vial size

0.08 µl, 0.4 µl

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the RAF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Jurkat cell lysate with RAF1 antibody. Predicted molecular weight ~73 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human HeLa cells with RAF1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with RAF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with RAF1 antibody; Blue=isotype control, Green= RAF1 antibody.