GTX103797

antibody from GeneTex

Targeting: PAK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX103797

Provider

GeneTex

Proper citation

GeneTex Cat#GTX103797, RRID:AB_10618667

Product name

PAK1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

p21-activated kinase 1 activity is required for histone H3 Ser¹⁰ phosphorylation and chromatin condensation in mouse oocyte meiosis.

Zhang N, Li X, Liu X, Cao Y, Chen D, Liu X, Wang Q, Du J, Weng J, Ma W
Reproduction, fertility, and development 2017 Jul;29(7):1287-1296

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE GTX103797 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PAK1 antibody detects PAK1 protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extract7.5% SDS-PAGEPAK1 antibody (GTX103797) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PAK1 antibody detects PAK1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract 7.5% SDS-PAGEPAK1 antibody (GTX103797) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody (GTX103797) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PAK1 antibody detects PAK1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: PAK1 protein stained by PAK1 antibody (GTX103797) diluted at 1:500.Blue: Hoechst 33343 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PAK1 antibody detects PAK1 protein on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PAK1 antibody (GTX103797) dilution: 1:500.