A00454

antibody from Boster Biological Technology

Targeting: PAK1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00454

Provider

Boster Biological Technology

Product name

Anti-PAK1 (Ab-212) Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for Q13153 detection. Tested positive for WB IHC IF in Hu Ms Rt

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at -20°C for long term preservation (recommended) . Store at 4°C for short term use.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of PAK1 using anti-PAK1 antibody (A00454).PAK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PAK1 Antibody (A00454) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image