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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-PW8140-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- Proteasome 20S _1 subunit monoclonal antibody (MCP421)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Protein G-affinity purified ascites.
- Reactivity
- Human, Rabbit, Yeast
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MCP421
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes.
Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux.
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells.
Purification and separation of the 20S immunoproteasome from the constitutive proteasome and identification of the subunits by LC-MS.
Bortezomib resistance in a myeloma cell line is associated to PSMβ5 overexpression and polyploidy.
Homma T, Kurahashi T, Lee J, Kang ES, Fujii J
Archives of biochemistry and biophysics 2015 Oct 1;583:65-72
Archives of biochemistry and biophysics 2015 Oct 1;583:65-72
Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux.
Bollinger LM, Powell JJ, Houmard JA, Witczak CA, Brault JJ
Obesity (Silver Spring, Md.) 2015 Jun;23(6):1185-93
Obesity (Silver Spring, Md.) 2015 Jun;23(6):1185-93
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells.
Hamouda MA, Belhacene N, Puissant A, Colosetti P, Robert G, Jacquel A, Mari B, Auberger P, Luciano F
Oncotarget 2014 Aug 15;5(15):6252-66
Oncotarget 2014 Aug 15;5(15):6252-66
Purification and separation of the 20S immunoproteasome from the constitutive proteasome and identification of the subunits by LC-MS.
Dechavanne V, Vilbois F, Glez L, Antonsson B
Protein expression and purification 2013 Feb;87(2):100-10
Protein expression and purification 2013 Feb;87(2):100-10
Bortezomib resistance in a myeloma cell line is associated to PSMβ5 overexpression and polyploidy.
Balsas P, Galán-Malo P, Marzo I, Naval J
Leukemia research 2012 Feb;36(2):212-8
Leukemia research 2012 Feb;36(2):212-8
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of Proteasome 20S _1 subunit, mAb (MCP421) (Prod. No. BML-PW8140): Lane 1: MW marker, Lane 2: HeLa (Prod. No. ADI-LYC-HL100), Lane 3: HeLa S100 fraction (Prod. No. BML-SW8750), Lane 4: 3T3 (Prod. No. ADI-LYC-3T100).
