bs-4294R

antibody from Bioss

Targeting: BUB1 BUB1A, BUB1L, hBUB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-4294R

Provider

Bioss

Proper citation

Bioss Cat#bs-4294R, RRID:AB_11099454

Product name

Bub1 Polyclonal Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic peptide derived from human Bub1

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Lane 1: mouse liver lysates Lane 2: mouse brain lysates probed with Anti Bub1 Polyclonal Antibody, Unconjugated (bs-4294R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 119kD. Observed band size: 119kD.

Sample type

Mouse

Other comments

Brain

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Bub1 Polyclonal Antibody, Unconjugated (bs-4294R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Human

Other comments

Colon