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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX107497 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107497, RRID:AB_1949770
- Product name
- BUB1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion.
Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Rodriguez-Rodriguez JA, Lewis C, McKinley KL, Sikirzhytski V, Corona J, Maciejowski J, Khodjakov A, Cheeseman IM, Jallepalli PV
Current biology : CB 2018 Nov 5;28(21):3422-3429.e5
Current biology : CB 2018 Nov 5;28(21):3422-3429.e5
Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Zhou H, Wang T, Zheng T, Teng J, Chen J
Nature communications 2016 Jan 8;7:10151
Nature communications 2016 Jan 8;7:10151
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX107497 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BUB1 antibody detects BUB1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract5 % SDS-PAGEBUB1 antibody (GTX107497) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using BUB1(GTX107497) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
