Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00000699-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000699-M01, RRID:AB_537237
- Product name
- Mouse Monoclonal Bub1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BUB1 - BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bub1 Antibody (4C3-4C9) [H00000699-M01] - Analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M01), clone 4C3-4C9.Lane 1: BUB1 transfected lysate(122.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Bub1 Antibody (4C3-4C9) [H00000699-M01] - Detection limit for recombinant GST tagged BUB1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Bub1 Antibody (4C3-4C9) [H00000699-M01] - Analysis of monoclonal antibody to BUB1 on HeLa cell . Antibody concentration 10 ug/ml.