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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB13273 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB13273, RRID:AB_10547558
- Product name
- BUB1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to C-terminus 22 amino acids of human BUB1.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Storage
- Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A dual role for Bub1 in the spindle checkpoint and chromosome congression.
Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint.
Mammalian BUB1 protein kinases: map positions and in vivo expression.
Meraldi P, Sorger PK
The EMBO journal 2005 Apr 20;24(8):1621-33
The EMBO journal 2005 Apr 20;24(8):1621-33
Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint.
Tang Z, Shu H, Oncel D, Chen S, Yu H
Molecular cell 2004 Nov 5;16(3):387-97
Molecular cell 2004 Nov 5;16(3):387-97
Mammalian BUB1 protein kinases: map positions and in vivo expression.
Pangilinan F, Li Q, Weaver T, Lewis BC, Dang CV, Spencer F
Genomics 1997 Dec 15;46(3):379-88
Genomics 1997 Dec 15;46(3):379-88
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BUB1 in A-20 cell lysate with BUB1 polyclonal antibody (Cat # PAB13273) at (A) 1 and (B) 2 ug/mL .
- Validation comment
- Western Blot (Cell lysate)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry of BUB1 in A-20 cells with BUB1 polyclonal antibody (Cat # PAB13273) at 5 ug/mL .
- Validation comment
- Immunocytochemistry
