Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-373A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bub1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Bub1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa treated with nacodazole (+) or mock treated (-), HEK293T. and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bub1 antibody (Product # A300-373A; lot A300-373A-2) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Bub1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bub1 (Cat. No. A300-373A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Bub1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with nocodazole. Antibodies: Affinity purified rabbit anti-Bub1 antibody (Product # A300-373A; lot A300-373A-2) used for IP at 6 µg per reaction. Bub1 was also immunoprecipitated by rabbit anti-Bub1 antibody BL1683. For blotting immunoprecipitated Bub1 (Product # A300-373A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
