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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Other assay [2]
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Validation data
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- Product number
- H00000699-M02 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BUB1 Monoclonal Antibody (2F9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MDTPENVLQM LEAHMQSYKG NDPLGEWERY IQWVEENFPE NKEYLITLLE HLMKEFLDKK KYHNDPRFIS YCLKFAEYNS DLHQFFEFLY NHGIGTLSSP LYIAWAGHLE AQGELQHASA VLQRGIQNQA
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F9
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M02), clone 2F9.Lane 1: BUB1 transfected lysate(122 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BUB1 monoclonal antibody (M02), clone 2F9 Western Blot analysis of BUB1 expression in HeLa S3 NE (Cat # L013V3).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged BUB1 is approximately 0.1 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of BUB1 transfected lysate using anti-BUB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BUB1 MaxPab rabbit polyclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of BUB1 transfected lysate using anti-BUB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BUB1 MaxPab rabbit polyclonal antibody.
