LS-C348732

antibody from LSBio

Targeting: ZBTB7A DKFZp547O146, FBI-1, LRF, pokemon, ZBTB7, ZNF857A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C348732

Provider

LSBio

Product name

Anti-ZBTB7A / Pokemon Antibody (clone BC17-2.9F) LS-C348732

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BC17-2.9F

Vial size

100µg

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot analysis using Pokemon Antibody (Clone BC17-2.9F) at 2 ug/ml on MDA-MD-231 breast cancer cell lysate. Secondary antibody was peroxidase conjugated Rabbit anti-Mouse Ig at 1:1000 dilution and developed by enhanced chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Nuclear and cytoplasmic staining of reactive tonsil (Human) is shown on Formalin Fixed Paraffin tissue. The sections were stained at 5 ug/ml with Pokemon Antibody (Clone BC17-2.9F) and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Nuclear staining of 1643 neuroblastoma cells is shown on Formalin Fixed Paraffin embedded tumor xenograft grown in mice. The sections were stained at 5 ug/ml with Pokemon Antibody (Clone BC17-2.9F) and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate. The high background reflects anti-Mouse secondary antibody also reacting against endogenous Mouse serum immunoglobulins present in the xenografts tissue.