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LearnAM32711PU-N
antibody from OriGene
Targeting: ZBTB7A
DKFZp547O146, FBI-1, LRF, pokemon, ZBTB7, ZNF857A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- AM32711PU-N - Provider product page
- Provider
- OriGene
- Product name
- Pokemon (ZBTB7A) mouse monoclonal antibody, clone BC17-2.9F, Purified
- Antibody type
- Monoclonal
- Description
- Pokemon (ZBTB7A) mouse monoclonal antibody, clone BC17-2.9F, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ZBTB7A
- Isotype
- IgG
- Antibody clone number
- BC17-2.9F
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis using Pokemon Antibody ?(Clone BC17-2.9F) at 2 ug/ml on MDA-MD-231 breast cancer cell lysate. Secondary antibody was peroxidase conjugated Rabbit anti-Mouse Ig at 1/1000 dilution and developed by enhanced chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Pokemon Antibody (Clone BC17-2.9F) staining of BT20, HCC1937, MDA-MB 436, SKBR3, TD47 Human breast cancer cell lines and SKNSH Human neuroblastoma cells attached on Marienfeld Superior slides.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Nuclear, perinuclear and cytoplasmic staining of MDA-MB-231 Human breast carcinoma cells is shown. The cells were stained at 5 ug/ml with Pokemon Antibody ?(Clone BC17-2.9F)?and FITC conjugated anti- Mouse Ig secondary antibody. While Pokemon has a strong nuclear localization signal, the intense localization in perinuclear and cytoplasmic areas may reflect pathology and remains to be investigated.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Nuclear staining of 1643 neuroblastoma cells is shown on Formalin Fixed Paraffin embedded tumor xenograft grown in mice. The sections were stained at 5 ug/ml with Pokemon Antibody ?(Clone BC17-2.9F)?and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate. The high background reflects anti-Mouse secondary antibody also reacting against endogenous Mouse serum immunoglobulins present in the xenografts tissue.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Nuclear and cytoplasmic staining of reactive tonsil (Human) is shown on Formalin Fixed Paraffin tissue. The sections were stained at 5 ug/ml with Pokemon Antibody ?(Clone BC17-2.9F) and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate.
- Validation comment
- IHC
