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LearnNB100-761
antibody from Novus Biologicals
Targeting: ZBTB7A
DKFZp547O146, FBI-1, LRF, pokemon, ZBTB7, ZNF857A
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-761 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-761, RRID:AB_2217243
- Product name
- Rabbit Polyclonal ZBTB7A/Pokemon Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Hypoxia-induced RelA/p65 derepresses SLC16A3 (MCT4) by downregulating ZBTB7A.
Leukemia/lymphoma-related factor regulates oligodendrocyte lineage cell differentiation in developing white matter.
Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers.
Critical roles of DMP1 in human epidermal growth factor receptor 2/neu-Arf-p53 signaling and breast cancer development.
Choi SH, Kim MY, Yoon YS, Koh DI, Kim MK, Cho SY, Kim KS, Hur MW
Biochimica et biophysica acta. Gene regulatory mechanisms 2019 Aug;1862(8):771-785
Biochimica et biophysica acta. Gene regulatory mechanisms 2019 Aug;1862(8):771-785
Leukemia/lymphoma-related factor regulates oligodendrocyte lineage cell differentiation in developing white matter.
Dobson NR, Moore RT, Tobin JE, Armstrong RC
Glia 2012 Sep;60(9):1378-90
Glia 2012 Sep;60(9):1378-90
Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers.
Aggarwal A, Hunter WJ 3rd, Aggarwal H, Silva ED, Davey MS, Murphy RF, Agrawal DK
Experimental and molecular pathology 2010 Oct;89(2):140-8
Experimental and molecular pathology 2010 Oct;89(2):140-8
Critical roles of DMP1 in human epidermal growth factor receptor 2/neu-Arf-p53 signaling and breast cancer development.
Taneja P, Maglic D, Kai F, Sugiyama T, Kendig RD, Frazier DP, Willingham MC, Inoue K
Cancer research 2010 Nov 15;70(22):9084-94
Cancer research 2010 Nov 15;70(22):9084-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - HeLa whole cell lysate using NB100-761. ZBTB7(FBI-1) was also immunoprecipitated using rabbit antibodies Another ZBTB7(FBI-1) Ab and NB100-762.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761] - Detection of human ZBTB7/FBI-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody NB100-761 (lot NB100-761-3) used for IP at 3 ug per reaction. ZBTB7/FBI-1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-761-2) and rabbit anti-ZBTB7/FBI-1 antibody NB100-762. For blotting immunoprecipitated ZBTB7/FBI-1, NB100-761 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
