PA5-55996

antibody from Invitrogen Antibodies

Targeting: UBE2E2 FLJ25157, UbcH8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55996

Provider

Invitrogen Antibodies

Product name

UBE2E2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: STEAQRVDDS PSTSGGSSDG DQRESVQQEP EREQVQPKKK EGKISSKTAA KLSTSAKRIQ KELAEITLDP PPNCSAGPKG DNIYEWRSTI LGPPGSVYEG GVFFLDITFS PDYPFKPPKV TFRTRIYHC

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UBE2E2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an UBE2E2 Polyclonal Antibody (Product # PA5-55996).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UBE2E2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an UBE2E2 Polyclonal Antibody (Product # PA5-55996).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of UBE2E2 in human cell line U-2 OS using an UBE2E2 Polyclonal Antibody (Product # PA5-55996) shows localization to nucleoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of UBE2E2 in human rectal tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using an UBE2E2 Polyclonal Antibody (Product # PA5-55996).