Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- C22-65R - Provider product page
- Provider
- SignalChem
- Product name
- Anti-phospho-CDK1 (Tyr15)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Purified by affinity chromatography
- Reactivity
- Human, Mouse, Rat, Xenopus, Zebrafish
- Host
- Rabbit
- Epitope
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Supportive validation
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- SignalChem (provider)
- Main image
- Experimental details
- Western blot of human T47D cells showing specific immunolabeling of the ~34kDa CDK1 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on CDK1 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: lambda-Ptase). These blots are identical to the control except that they were incubated in lambda-Ptase (1200 units for 30 min) before being exposed to Anti-phospho-CDK1 (Tyr15). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
- Sample type
- Western Blot