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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX108120 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108120, RRID:AB_1949938
- Product name
- CDC2 antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.
A high-throughput pipeline for validation of antibodies.
The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.
Intrahepatic hepatitis B virus large surface antigen induces hepatocyte hyperploidy via failure of cytokinesis.
Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Escin induces apoptosis in human renal cancer cells through G2/M arrest and reactive oxygen species-modulated mitochondrial pathways.
Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro.
Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Ko PH, Shen YC, Murugan K, Huang CW, Sivakumar G, Pal P, Liao CC, Luo KS, Chuang EY, Tsai MH, Lai LC
Scientific reports 2019 Feb 14;9(1):2147
Scientific reports 2019 Feb 14;9(1):2147
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.
Huang TH, Fowler F, Chen CC, Shen ZJ, Sleckman B, Tyler JK
Molecular cell 2018 Mar 1;69(5):879-892.e5
Molecular cell 2018 Mar 1;69(5):879-892.e5
Intrahepatic hepatitis B virus large surface antigen induces hepatocyte hyperploidy via failure of cytokinesis.
Li TN, Wu YJ, Tsai HW, Sun CP, Wu YH, Wu HL, Pei YN, Lu KY, Yen TT, Chang CW, Chan HL, Tao MH, Liou JY, Chang MD, Su IJ, Wang LH
The Journal of pathology 2018 Aug;245(4):502-513
The Journal of pathology 2018 Aug;245(4):502-513
Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.
Lu WJ, Wu GJ, Chen RJ, Chang CC, Lien LM, Chiu CC, Tseng MF, Huang LT, Lin KH
Food & function 2018 Aug 15;9(8):4500-4507
Food & function 2018 Aug 15;9(8):4500-4507
Escin induces apoptosis in human renal cancer cells through G2/M arrest and reactive oxygen species-modulated mitochondrial pathways.
Yuan SY, Cheng CL, Wang SS, Ho HC, Chiu KY, Chen CS, Chen CC, Shiau MY, Ou YC
Oncology reports 2017 Feb;37(2):1002-1010
Oncology reports 2017 Feb;37(2):1002-1010
Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro.
Chin YT, Hsieh MT, Yang SH, Tsai PW, Wang SH, Wang CC, Lee YS, Cheng GY, HuangFu WC, London D, Tang HY, Fu E, Yen Y, Liu LF, Lin HY, Davis PJ
Oncotarget 2014 Dec 30;5(24):12891-907
Oncotarget 2014 Dec 30;5(24):12891-907
Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Cheung AK, Ip JC, Lung HL, Wu JZ, Tsao SW, Lung ML
Molecular cancer therapeutics 2013 Aug;12(8):1393-401
Molecular cancer therapeutics 2013 Aug;12(8):1393-401
Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Chu CS, Hsu PH, Lo PW, Scheer E, Tora L, Tsai HJ, Tsai MD, Juan LJ
The Journal of biological chemistry 2011 Oct 14;286(41):35843-51
The Journal of biological chemistry 2011 Oct 14;286(41):35843-51
Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2.
Pan J, Nakade K, Huang YC, Zhu ZW, Masuzaki S, Hasegawa H, Murata T, Yoshiki A, Yamaguchi N, Lee CH, Yang WC, Tsai EM, Obata Y, Yokoyama KK
Oncogene 2010 Nov 25;29(47):6245-56
Oncogene 2010 Nov 25;29(47):6245-56
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE GTX108120 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE GTX108120 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: PC-12 10% SDS PAGE GTX108120 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 antibody [N1], N-term (GTX108120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis.Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.Green: CDC2 protein stained by CDC2 antibody [N1], N-term (GTX108120) diluted at 1:750.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CDC2 antibody [N1], N-term (GTX108120) diluted at 1:500.
