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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX110545 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110545, RRID:AB_10721710
- Product name
- CDC2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Submitted references A high-throughput pipeline for validation of antibodies.
Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Liu YH, Weng YP, Tsai HY, Chen CJ, Lee DY, Hsieh CL, Wu YC, Lin JY
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192
Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437
Scientific reports 2017 Jan 30;7:41437
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE GTX110545 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC2 antibody detects CDC2 protein by Western blot analysis.A. 30 μg GL261 whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract 10 % SDS-PAGECDC2 antibody (GTX110545) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using CDC2(GTX110545) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of CDC2 protein from A431 whole cell extracts using 5 £gg of CDC2 antibody (GTX110545).Western blot analysis was performed using CDC2 antibody (GTX110545).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
