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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000983-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000983-M01, RRID:AB_425355
- Product name
- CDC2 monoclonal antibody (M01), clone 1A4-1A9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CDC2.
- Antigen sequence
MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKI
RLESEEEGVPSTAIREISLLKELRHPNIVSLQDVL
MQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLV
KSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKG
TIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVL
LGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEI
DQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWK
PGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKM
ALNHPYFNDLDNQIKKM- Isotype
- IgG
- Antibody clone number
- 1A4-1A9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDC2 monoclonal antibody (M01), clone 1A4-1A9 Western Blot analysis of CDC2 expression in Hela ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.Lane 1: CDC2 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CDC2 is approximately 30ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
