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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0436 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0436, RRID:AB_982433
- Product name
- CDC2 (phospho S39) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase.
Mitotic phosphorylation of the peripheral Golgi protein Nir2 by Cdk1 provides a docking mechanism for Plk1 and affects cytokinesis completion.
Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
Krämer A, Mailand N, Lukas C, Syljuåsen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J
Nature cell biology 2004 Sep;6(9):884-91
Nature cell biology 2004 Sep;6(9):884-91
Mitotic phosphorylation of the peripheral Golgi protein Nir2 by Cdk1 provides a docking mechanism for Plk1 and affects cytokinesis completion.
Litvak V, Argov R, Dahan N, Ramachandran S, Amarilio R, Shainskaya A, Lev S
Molecular cell 2004 May 7;14(3):319-30
Molecular cell 2004 May 7;14(3):319-30
Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
Dai X, Yamasaki K, Yang L, Sayama K, Shirakata Y, Tokumara S, Yahata Y, Tohyama M, Hashimoto K
The Journal of investigative dermatology 2004 Jun;122(6):1356-64
The Journal of investigative dermatology 2004 Jun;122(6):1356-64
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436) is used in Western blot for detection in Ramos cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436). Western blot analysis of CDK1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of CDC2 (phospho S39) polyclonal antibody (Cat # PAB0436) on nitrocellulose membrane. 50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
