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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-401-160 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-160, RRID:AB_2074799
- Product name
- Anti-cdc2 (p34) (RABBIT) Antibody - 100-401-160
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Rockland's anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1?uM, 6.2?uM, 12.5?uM and 25.0?uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1,000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
- Validation comment
- Western Blot
