MA5-11472

antibody from Invitrogen Antibodies

Targeting: CDK1 CDC2, CDC28A

Immunoprecipitation

Antibody data

Submit

Validation data

CDK1 protein structure - MA5-11472 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cdk1 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk1 monoclonal antibody (Product # MA5-11472) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cdk1 (green) showing staining in the cytoplasm of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk1 monoclonal antibody (Product # MA5-11472) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cdk1 (green) showing staining in the cytoplasm of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk1 monoclonal antibody (Product # MA5-11472) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Cdk1 using Cdk1 Monoclonal Antibody (Product # MA5-11472) on denatured Human HeLa Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Cdk1 using Cdk1 Monoclonal Antibody (Product # MA5-11472) on Native Human HeLa Cells.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Treatment of BI6727 and GSK461364 altered expression of cell cycle regulators. (a) Time course analysis of CCNB1 and CDK1 as well as phosphorylated CDK1 (Y15) protein levels was performed by western blot analysis. For loading control, the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used. (b) Changes in CCNB1 expression were validated on messenger RNA level by semi-quantitative polymerase chain reaction. Polymerase chain reactions are normalized to GAPDH and calibrated to untreated cells. Bars represent the mean of three independent experiments. Error bars indicate standard error of the mean. *** P < 0.001, ** P = 0.001-0.01, * P > 0.01-0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIG 6 Upregulated proteins are enriched in cell cycle functions. (A) DAVID enrichment analysis of proteins upregulated or downregulated >2-fold against a background of all 8,985 human proteins quantified in experiment 1. No significantly enriched downregulated clusters were observed for HU cells. (B) Example protein profiles for selected cell cycle-related proteins for both RPTE and HU cells. Protein families are separated by colored boxes. (C) Validation of selected temporal profiles shown in panel B by Western blotting (RPTE cells; MOI of 3). Tubulin was used as a loading control, and VP1 was used as a control for infection.