- GTX109519 - GeneTex MAD1L1 antibody

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The international journal of biochemistry & cell biology 2018 Jan;94:22-30

Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.

Shirnekhi HK, Kelley EP, DeLuca JG, Herman JA
Molecular biology of the cell 2017 Jul 15;28(15):2035-2041

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Nature communications 2016 Jan 8;7:10151

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Yeh PC, Yeh CC, Chen YC, Juang YL
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Supportive validation

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Main image
Experimental details: Sample (30 ?g of whole cell lysate)A: Molt-4 (GTX27912)7.5% SDS PAGEGTX109519 diluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MAD1 antibody detects MAD1L1 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g NIH-3T3 whole cell lysate/extract E. 30 ?g BCL-1 whole cell lysate/extract F. 30 ?g Raw264.7 whole cell lysate/extract 7.5% SDS-PAGEMAD1 antibody (GTX109519) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MAD1 antibody detects MAD1L1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract7.5% SDS-PAGEMAD1 antibody (GTX109519) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MAD1 antibody detects MAD1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody (GTX109519) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MAD1 antibody detects MAD1 protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody (GTX109519) diluted by 1:5000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MAD1 antibody detects MAD1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAD1 protein stained by MAD1 antibody (GTX109519) diluted at 1:500.Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] (GTX70268) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 £gg of MAD1 antibody (GTX109519).Western blot analysis was performed using MAD1 antibody (GTX109519).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using Mad 1 (GTX109519) antibody at 1:100 dilution.

GTX109519

Antibody data