PAB20232

antibody from Abnova Corporation

Targeting: MAD1L1 HsMAD1, MAD1, PIG9, TP53I9, TXBP181

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20232

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20232, RRID:AB_10961968

Product name

MAD1L1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MAD1L1.

Antigen sequence

TKVLHMSLNPTSVARQRLREDHSQLQAECERLRGL
LRAMERGGTVPADLEAAAASLPSSKEVAELKKQVE
SAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDIT
TENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLE
IRRAPPLSNN


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MAD1L1 polyclonal antibody (Cat # PAB20232) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with MAD1L1 polyclonal antibody (Cat # PAB20232) at 1-4 ug/mL dilution shows positivity in nucleus, cytoskeleton (actin filaments).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with MAD1L1 polyclonal antibody (Cat # PAB20232) shows strong nuclear positivity in neuronal cells and glial cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)