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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 12251-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDLPTCGAR NLKQHLAKAS ASGIMGSPKP AIKSISASAL LKQQKQRMLE MRRRKSEEIQ KRFLQSSSEV ESPAVPSSSR QPPAQPPRTG SEFPRLEGAP ATMTPKLGRG VLEGDDVLFY DESPPPRPKL SALAEAKKLA AITKLRAKGQ VLTKTNPNSI KKKQKDPQDI LEVKERVEKN TMFSSQAEDE LEPARKKRRE QLAYLESEEF QKILKAKSKH TGILKEAEAE MQERYFEPLV KKEQMEEKMR NIREVKCRVV TCKTCAYTHF KLLETCVSEQ HEYHWHDGVK RFFKCPCGNR SISLDRLPNK HCSNCGLYKW ERDGMLKEKT GPKIGGETLL PRGEEHAKFL NSLK (259-611 aa encoded by B C009108)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 12251-1-AP (MCM10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12251-1-AP (MCM10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
