Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-253 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-253, RRID:AB_2281823
- Product name
- Rabbit Polyclonal MCM10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Singlet Oxygen-Mediated Oxidation during UVA Radiation Alters the Dynamic of Genomic DNA Replication.
Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells: DNA-binding activity and novel protein interactions.
Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication.
The Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism.
Graindorge D, Martineau S, Machon C, Arnoux P, Guitton J, Francesconi S, Frochot C, Sage E, Girard PM
PloS one 2015;10(10):e0140645
PloS one 2015;10(10):e0140645
Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells: DNA-binding activity and novel protein interactions.
Uno S, Masai H
Genes to cells : devoted to molecular & cellular mechanisms 2011 Aug;16(8):842-56
Genes to cells : devoted to molecular & cellular mechanisms 2011 Aug;16(8):842-56
Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication.
Chattopadhyay S, Bielinsky AK
Molecular biology of the cell 2007 Oct;18(10):4085-95
Molecular biology of the cell 2007 Oct;18(10):4085-95
The Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism.
Liu P, Barkley LR, Day T, Bi X, Slater DM, Alexandrow MG, Nasheuer HP, Vaziri C
The Journal of biological chemistry 2006 Oct 13;281(41):30631-44
The Journal of biological chemistry 2006 Oct 13;281(41):30631-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM10 Antibody [NB100-253] - Detection of Human MCM10 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody NB100-253 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM10 Antibody [NB100-253] - Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody NB100-253 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM10 Antibody [NB100-253] - No relocalization or post-translational modifications of some components of the replication machinery after UVA radiation. Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. Subcellular localization of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, Mcm10, Orc2, PCNA, RPA32 in asynchronous cells (Asyncro.) or cells synchronized in early S-phase (Synchro.). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM10 Antibody [NB100-253] - Asynchronous MRC5Vi cells or synchronized in early S-phase (condition S0R, see Material and Methods) are untreated or exposed to 80 and 160 kJ/m2 of UVA radiation. The fractions of chromatin-bound proteins and total soluble proteins were recovered at various time points post UVA. The expression levels of Cdc6, Cdc7, Dbf4, Cdc45, Mcm2, p-Mcm2(Ser40/41), Mcm10, Orc2, PCNA, and RPA32 were detected by Western blotting in denaturing and reducing conditions. Lamin A/C and GAPDH were used as loading control for the chromatin-bound and soluble fractions, respectively. The apparent molecular weight of each protein is indicated on the right side of each blot (panel 7A). The stars (*) indicate the position of the non specific bands detected by Orc2 antibody. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140645), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MCM10 Antibody [NB100-253] - HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT116 cells (not shown). Antibody: Affinity purified rabbit anti- NB100-253 used at 1 ug/mg lysate for IP and 0.5 ug/ml for WB. Detection: Chemiluminescence.