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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1951 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1951, RRID:AB_1574203
- Product name
- DAAM1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DAAM1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila.
Mechanism of activation of the Formin protein Daam1.
Crystal structure of human DAAM1 formin homology 2 domain.
Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.
Molnár I, Migh E, Szikora S, Kalmár T, Végh AG, Deák F, Barkó S, Bugyi B, Orfanos Z, Kovács J, Juhász G, Váró G, Nyitrai M, Sparrow J, Mihály J
PLoS genetics 2014 Feb;10(2):e1004166
PLoS genetics 2014 Feb;10(2):e1004166
Mechanism of activation of the Formin protein Daam1.
Liu W, Sato A, Khadka D, Bharti R, Diaz H, Runnels LW, Habas R
Proceedings of the National Academy of Sciences of the United States of America 2008 Jan 8;105(1):210-5
Proceedings of the National Academy of Sciences of the United States of America 2008 Jan 8;105(1):210-5
Crystal structure of human DAAM1 formin homology 2 domain.
Yamashita M, Higashi T, Suetsugu S, Sato Y, Ikeda T, Shirakawa R, Kita T, Takenawa T, Horiuchi H, Fukai S, Nureki O
Genes to cells : devoted to molecular & cellular mechanisms 2007 Nov;12(11):1255-65
Genes to cells : devoted to molecular & cellular mechanisms 2007 Nov;12(11):1255-65
Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.
Lu J, Meng W, Poy F, Maiti S, Goode BL, Eck MJ
Journal of molecular biology 2007 Jun 22;369(5):1258-69
Journal of molecular biology 2007 Jun 22;369(5):1258-69
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of DAAM1 (arrow) using DAAM1 polyclonal antibody (Cat # PAB1951). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 polyclonal antibody (Cat # PAB1951) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
