Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00023002-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023002-M03, RRID:AB_2089446
- Product name
- Mouse Monoclonal DAAM1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DAAM1 (4H3)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references MTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cells.
Kawabata Galbraith K, Fujishima K, Mizuno H, Lee SJ, Uemura T, Sakimura K, Mishina M, Watanabe N, Kengaku M
Cell reports 2018 Jul 3;24(1):95-106.e9
Cell reports 2018 Jul 3;24(1):95-106.e9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M03), clone 4H3. Lane 1: DAAM1 transfected lysatE (122.3 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in A-431 (Cat # L015V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DAAM1 Antibody (4H3) [H00023002-M03] - Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.