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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-10759 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-10759, RRID:AB_2210610
- Product name
- Anti-UBE2I
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references SUMO E3 ligase activity of TRIM proteins.
The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation.
Spi-1 and Fli-1 directly activate common target genes involved in ribosome biogenesis in Friend erythroleukemic cells.
Chu Y, Yang X
Oncogene 2011 Mar 3;30(9):1108-16
Oncogene 2011 Mar 3;30(9):1108-16
The human COP9 signalosome protects ubiquitin-conjugating enzyme 3 (UBC3/Cdc34) from beta-transducin repeat-containing protein (betaTrCP)-mediated degradation.
Fernandez-Sanchez ME, Sechet E, Margottin-Goguet F, Rogge L, Bianchi E
The Journal of biological chemistry 2010 Jun 4;285(23):17390-7
The Journal of biological chemistry 2010 Jun 4;285(23):17390-7
Spi-1 and Fli-1 directly activate common target genes involved in ribosome biogenesis in Friend erythroleukemic cells.
Juban G, Giraud G, Guyot B, Belin S, Diaz JJ, Starck J, Guillouf C, Moreau-Gachelin F, Morlé F
Molecular and cellular biology 2009 May;29(10):2852-64
Molecular and cellular biology 2009 May;29(10):2852-64
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 20
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
