Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM06691SU-N - Provider product page
- Provider
- OriGene
- Product name
- UBE2I mouse monoclonal antibody, clone 1B10, Ascites
- Antibody type
- Monoclonal
- Description
- UBE2I mouse monoclonal antibody, clone 1B10, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UBE2I
- Isotype
- IgG
- Antibody clone number
- 1B10
- Vial size
- 100 µl
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using UBE2I mAb against human UBE2I (AA: 1-158) recombinant protein. (Expected MW is 45.3 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).
- Validation comment
- FC
