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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB300-812 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-812, RRID:AB_2288055
- Product name
- Goat Polyclonal UBE2I/Ubc9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Depleting Trim28 in adult mice is well tolerated and reduces levels of α-synuclein and tau.
UBC9 regulates the stability of XBP1, a key transcription factor controlling the ER stress response.
UBC9 regulates the stability of XBP1, a key transcription factor controlling the ER stress response.
Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
Rousseaux MW, Revelli JP, Vázquez-Vélez GE, Kim JY, Craigen E, Gonzales K, Beckinghausen J, Zoghbi HY
eLife 2018 Jun 4;7
eLife 2018 Jun 4;7
UBC9 regulates the stability of XBP1, a key transcription factor controlling the ER stress response.
Uemura A, Taniguchi M, Matsuo Y, Oku M, Wakabayashi S, Yoshida H
Cell structure and function 2013;38(1):67-79
Cell structure and function 2013;38(1):67-79
UBC9 regulates the stability of XBP1, a key transcription factor controlling the ER stress response.
Uemura A, Taniguchi M, Matsuo Y, Oku M, Wakabayashi S, Yoshida H
Cell structure and function 2013;38(1):67-79
Cell structure and function 2013;38(1):67-79
Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF
The Journal of biological chemistry 1996 Oct 4;271(40):24811-6
The Journal of biological chemistry 1996 Oct 4;271(40):24811-6
Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF
The Journal of biological chemistry 1996 Oct 4;271(40):24811-6
The Journal of biological chemistry 1996 Oct 4;271(40):24811-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Rat Ovary lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Staining of Human Kidney. Antibody at 0.3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NB300-812] - Human breast cancer tissue. IHC-P image submitted by a verified customer review.
