PA5-17215

antibody from Invitrogen Antibodies

Targeting: SIRT6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17215

Provider

Invitrogen Antibodies

Product name

SIRT6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Concentration

16 µg/mL

References

Submitted references

Natural polyphenols as sirtuin 6 modulators.

Rahnasto-Rilla M, Tyni J, Huovinen M, Jarho E, Kulikowicz T, Ravichandran S, A Bohr V, Ferrucci L, Lahtela-Kakkonen M, Moaddel R
Scientific reports 2018 Mar 7;8(1):4163

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Myeloid deletion of SIRT1 induces inflammatory signaling in response to environmental stress.

Schug TT, Xu Q, Gao H, Peres-da-Silva A, Draper DW, Fessler MB, Purushotham A, Li X
Molecular and cellular biology 2010 Oct;30(19):4712-21

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of SIRT6 was achieved by transfecting A549 cells with SIRT6 specific siRNAs (Silencer® select Product # s195228, s28306). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the SIRT6 knockdown cells (lane 2) and non-target siRNA transfected cells (lane 1). The blot was probed with SIRT6 Polyclonal Antibody (Product # PA5-17215, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SIRT6.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SirT6 in Hela and Jurkat cells using SirT6 polyclonal antibody (Product # PA5-17215).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1), MCF7 (Lane 2), HCT 116 (Lane 3), Jurkat (Lane 4), HeLa (Lane 5), iPSC (Lane 6) and Embryoid Bodies (Lane 7). The blot was probed with Anti-SIRT6 Polyclonal Antibody (Product # PA5-17215, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 45 kDa band corresponding to SIRT6 was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SIRT6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SIRT6 Polyclonal Antibody (Product # PA5-17215) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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