PAB29394

antibody from Abnova Corporation

Targeting: WSB1 DKFZp564A122, DKFZp564B0482, SWIP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29394

Provider

Abnova Corporation

Product name

WSB1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human WSB1.

Antigen sequence

APFDKKCGRENWTVAFAPDGSYFAWSQGHRTVKLV
PWSQCLQNFLLHGTKNVTNSSSLRLPRQNSDGGQK
NKPREHIIDCGDIVWSLAFGSSVPEKQSRCVNIEW
HRFRFGQDQLLLATGLNNGRIKIWDV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with WSB1 polyclonal antibody (Cat # PAB29394) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with WSB1 polyclonal antibody (Cat # PAB29394) at 1-4 ug/mL concentration shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with WSB1 polyclonal antibody (Cat # PAB29394) shows cytoplasmic and membranous positivity in hepatocytes at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)